Alumnae Biographical Files primarily contain biographical questionnaires, correspondence, articles and other publications, press releases, and notes documenting the lives of Mount Holyoke alumnae.. Also include diaries, letters, notebooks, and other personal materials for these individuals, often describing their experiences as students. Topics discussed by alumnae in these materials include their personal lives and employment in teaching, missionary work, and other fields. There is also extensive information about the curriculum, teachers and administrators (including Mary Lyon), regulations, schedules, expenses, domestic work system, books and reading, exercise and physical education program, religious life, traditions, Thanksgiving and other holiday celebrations, food, care of clothing, and buildings and grounds at Mount Holyoke.

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Use of the collection is governed by the Copyright Law of the United States (Title 17, U.S.C.)